Research Progress of Environmental RNA Technology in Bio-monitoring
Ecosystems on a global scale are facing unprecedented loss of biodiversity and changes in community structure.In order to detect these changes in a timely and accurate manner,scientists are constantly exploring new monitoring technologies.Among them,environmental DNA(eDNA)technology has been widely used as a non-invasive method in biological resource assessment and biodiversity surveys.However,it may lead to false positive results and affect the accuracy of monitoring.In contrast,environmental RNA(eRNA)technol-ogy can more effectively distinguish living(metabolically active)organism or dead(dormant)organisms,re-duce false positive results,and thus improve the reliability of monitoring.However,eRNA technology has not yet been well known,especially in China,where it is rarely used.The purpose of this paper is to review the re-search status of eRNA technology in biomonitoring,and compare it with traditional monitoring methods and eDNA technology to explore its advantages,limitations and existing problems.At the same time,the research progress of eRNA in different aquatic ecosystems is analyzed by bibliometrics,and the applicability and feasi-bility of eRNA technology in ecological monitoring in the future were prospected.