Effects of Simulated Nitrogen Deposition on Diversities and Community Structures of Soil Bacteria in Subtropical Evergreen Broad-leaved Forests of Mao'er Mountain
In order to understand change laws of soil bacterial communities in Mao'er mountain under atmo-spheric nitrogen deposition,taking subtropical evergreen broad-leaved forests of Mao'er mountain as research objects,four different nitrogen application treatments[0(CK)、50(Ⅱ)、100(Ⅲ)and 150(Ⅳ)g·m-2·a-1]were set up.Effects of different nitrogen applications on diversity and community structure of soil bacteria were stud-ied by high-throughput sequencing technology.Results showed that treatment Ⅲ had the highest number of unique soil bacterial OTUs.Simpson index decreased with increase of nitrogen application.Treatment Ⅱ had the highest Simpson index.Shannon and Chao 1 indexes showed trends of increase firstly and then decrease with increase of nitrogen application.TreatmentⅢhad the highest Shannon and Chao 1 indexes.Soil bacterial communities were dominated by Acidobacteria,Proteobacteria,Chloroflexi,Firmicutes,Verrucomicrobia and Actinobacteria.NMDS and cluster analysis showed that different nitrogen application treatments did not result in obvious separation of bacterial community structure.LDA analysis showed that treatmentⅢhad the highest number of differentiated species.Chloroflexi could be used as indicator species after nitrogen application.100 g·m-2·a-1 nitrogen application was the most beneficial to bacterial growth.150 g·m-2·a-1 nitrogen application had certain inhibitory effect on soil bacterial growth.
万方数据
维普
