摘要
为了解大气氮沉降下猫儿山土壤细菌群落的变化规律,以猫儿山亚热带常绿阔叶林为对象,设置4种不同施氮处理[0(CK)、50(Ⅱ)、100(Ⅲ)和150(Ⅳ)g·m-2·a-1],采用高通量测序技术研究不同施氮量对土壤细菌多样性和群落结构的影响.结果表明,处理Ⅲ的特有土壤细菌OTU数量最多.随施氮量增加,辛普森指数降低,处理Ⅱ的辛普森指数最高;随施氮量增加,香农和Chao 1指数均呈先升后降的趋势,处理Ⅲ的香农和Chao 1指数均最高.土壤细菌群落以酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)、疣微菌门(Verrucomicrobia)和放线菌门(Actinobacteria)为主.NMDS和聚类分析表明,不同施氮处理未造成细菌群落结构明显分离.LDA分析表明,处理Ⅲ的差异性物种数量最多,绿弯菌门可作为氮添加后的指示性物种.100 g·m-2·a-1施氮量最有利于土壤细菌生长,150 g·m-2·a-1施氮量对土壤细菌生长有一定抑制作用.
Abstract
In order to understand change laws of soil bacterial communities in Mao'er mountain under atmo-spheric nitrogen deposition,taking subtropical evergreen broad-leaved forests of Mao'er mountain as research objects,four different nitrogen application treatments[0(CK)、50(Ⅱ)、100(Ⅲ)and 150(Ⅳ)g·m-2·a-1]were set up.Effects of different nitrogen applications on diversity and community structure of soil bacteria were stud-ied by high-throughput sequencing technology.Results showed that treatment Ⅲ had the highest number of unique soil bacterial OTUs.Simpson index decreased with increase of nitrogen application.Treatment Ⅱ had the highest Simpson index.Shannon and Chao 1 indexes showed trends of increase firstly and then decrease with increase of nitrogen application.TreatmentⅢhad the highest Shannon and Chao 1 indexes.Soil bacterial communities were dominated by Acidobacteria,Proteobacteria,Chloroflexi,Firmicutes,Verrucomicrobia and Actinobacteria.NMDS and cluster analysis showed that different nitrogen application treatments did not result in obvious separation of bacterial community structure.LDA analysis showed that treatmentⅢhad the highest number of differentiated species.Chloroflexi could be used as indicator species after nitrogen application.100 g·m-2·a-1 nitrogen application was the most beneficial to bacterial growth.150 g·m-2·a-1 nitrogen application had certain inhibitory effect on soil bacterial growth.
基金项目
广西林业科技推广示范项目(2023GXLK13)
广西林科院基本科研业务费项目(林科201815号)
广西林业科技项目(桂林科研[2015]第34号)
维普
万方数据