Tissue Culture and Rapid Propagation System of Barthea barthei
In order to establish tissue culture and fast propagation system for Barthea barthei and promote rapid propagation of excellent germplasm resources of B.barthei,taking stem segments of B.barthei as explants,ef-fects of lignification degree of stem segments,remained leaf methods and disinfection time as well as culture mediums on tissue culture and fast propagation of B.barthei were studied.Results showed that stem segments of B.barthei had the highest survival rate(75.69%)by using 1/2MS+0.5 mg/L 6-BA as culture medium and se-lecting shoots as explants.Stem segments of B.barthei had the highest survival rate(91.67%)by retaining whole leaves,treated with 75%alcohol for 10 s and sterilized with 0.1%ascending mercury for 5 min.Optimum culture medium for primary culture of B.barthei was 1/2MS+0.5 mg/L 6-BA with the highest germination rate(83.33%).Optimum culture medium for proliferation culture of B.barthei was WPM+0.5 mg/L 6-BA with the highest proliferation coefficient(3.00).Optimum culture medium for rooting culture of B.barthei was 1/2 MS+1.0 mg/L IBA with the highest rooting rate(95.24%).Substrate formulation for B.barthei transplanting was coco coir∶peat soil∶perlite=3∶3∶1(V∶V∶V)with 85%survival rate.
万方数据
维普
