摘要
为建立棱果花(Barthea barthei)组培快繁体系,推动棱果花优良种质资源快速繁育进程,以棱果花优树茎段作为外植体,研究茎段木质化程度、叶片保留方式和灭菌时间及培养基对棱果花组培快繁的影响.结果表明,采用1/2MS+0.5 mg/L 6-BA作为培养基,选择嫩枝作为外植体,棱果花茎段存活率最高(75.69%).采用保留全叶方式,经75%酒精处理10 s,0.1%升汞灭菌5 min,棱果花茎段存活率最高(91.67%).棱果花初代培养的最佳培养基为1/2MS+0.5 mg/L 6-BA,萌芽率最高(83.33%).棱果花增殖培养的最佳培养基为WPM+0.5 mg/L 6-BA,增殖系数最高(3.00).棱果花生根培养的最佳培养基为1/2MS+1.0 mg/L IBA,生根率最高(95.24%).棱果花移栽基质配方为椰糠∶泥炭土∶珍珠岩=3∶3∶1(V∶V∶V)时,成活率达85%.
Abstract
In order to establish tissue culture and fast propagation system for Barthea barthei and promote rapid propagation of excellent germplasm resources of B.barthei,taking stem segments of B.barthei as explants,ef-fects of lignification degree of stem segments,remained leaf methods and disinfection time as well as culture mediums on tissue culture and fast propagation of B.barthei were studied.Results showed that stem segments of B.barthei had the highest survival rate(75.69%)by using 1/2MS+0.5 mg/L 6-BA as culture medium and se-lecting shoots as explants.Stem segments of B.barthei had the highest survival rate(91.67%)by retaining whole leaves,treated with 75%alcohol for 10 s and sterilized with 0.1%ascending mercury for 5 min.Optimum culture medium for primary culture of B.barthei was 1/2MS+0.5 mg/L 6-BA with the highest germination rate(83.33%).Optimum culture medium for proliferation culture of B.barthei was WPM+0.5 mg/L 6-BA with the highest proliferation coefficient(3.00).Optimum culture medium for rooting culture of B.barthei was 1/2 MS+1.0 mg/L IBA with the highest rooting rate(95.24%).Substrate formulation for B.barthei transplanting was coco coir∶peat soil∶perlite=3∶3∶1(V∶V∶V)with 85%survival rate.
维普
万方数据