Effects of Different Preservative Treatments on Preservative Effects and Physiological and Biochemical Indexes of Gardenia jasminoides var.fortuniana Cuttings
In order to prolong vase life of Gardenia jasminoides var.fortuniana cuttings and improve its orna-mental value,taking G.jasminoides var.fortuniana cuttings as experimental materials,using sucrose,8-hy-droxyquinoline(8-HQ)and 6-benzylaminoadenine(6-BA)as basic ingredients,methyl jasmonate(A),citric ac-id(B),aluminium potassium sulfate(C)and salicylic acid(D)solutions were added as preservatives respective-ly.Water was control(CK).Changes of appearances of G.jasminoides var.fortuniana cuttings were observed.Ef-fects of different preservative treatments on vase life and physiological and biochemical indexes of G.jasminoi-des var.fortuniana cuttings were compared.Optimal preservative treatment was screened.Results showed that different preservative treatments had significant effects on appearances,vase lives,change rates of fresh masses as well as physiological and biochemical indexes of cuttings.In D treatment,branch of cutting was straight,leaves were healthy,water retention ability was better,and vase life of cutting was 42 days.Followed by B treat-ment with vase life of cutting 40 days.Relative conductivities of cuttings in A,B,C and D treatments were low-er than CK.Contents of chlorophyll a,chlorophyll b and total chlorophyll of cutting were higher in C treatment.A,B,C and D treatments were able to significantly increase activities of superoxide dismutase(SOD)and cata-lase(CAT)of cuttings.Peroxidase(POD)activities of cuttings in A,B and C treatments had no significant differ-ences with CK.POD activity of cutting in D treatment was significantly lower than CK.Malondialdehyde(MDA)contents of cuttings were lower in B and D treatments.Soluble protein content of cutting in C treatment was the highest.Soluble sugar contents of cuttings in C and D treatments were higher.To sum up,D treatment(1%su-crose+200 mg/L 8-HQ+50 mg/L 6-BA+250 mg/L salicylic acid)had the best preservative effect on G.jasminoi-des var.fortuniana cuttings.