ISSR Analysis of Genetic Diversity of Ancient Tea Plant Resources in Guilin
[Objective]ISSR molecular marker technology was applied to study the genetic diversity and genetic relationship of 50 ancient tea resources in Guilin,in order to fully understand their genetic characteristics,and provide reference for the evaluation and identification of tea germplasm resources,germplasm resources screening,resource protection and utilization in Guilin.[Method]The genetic diversity of 50 ancient tea plant resources and 12 improved variety resources in Guilin was analyzed by 13 primers.[Result]Thirteen primers amplified 223 bands,210 bands were polymorphic,with a polymorphism ratio of 94.17%,indicating that Guilin ancient tea trees have rich genetic diversity.[Conclusion]Guilin ancient tree tea is mostly concentrated in Longsheng County-Ziyuan County-Xing'an County.There is a phenomenon of gene exchange and the genetic distance is relatively short.