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桂林古茶树资源遗传多样性的ISSR分析

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[目的]利用ISSR分子标记技术对桂林 50份古茶树资源的遗传多样性与亲缘关系进行研究,以期全面了解其基因特征,为桂林茶树种质资源评价与鉴定、种质资源筛选、资源保护与利用等提供参考.[方法]用 13 条引物对桂林 50 份古茶树单株资源和 12 份良种资源的遗传多样性进行分析.[结果]13 条引物扩增出 223 条条带,210 条条带具有多态性,多态性比率为 94.17%,表明桂林古茶树具有丰富的遗传多样性.[结论]桂林古茶树多集中在龙胜各族自治县—资源县—兴安县一带,存在基因交流现象,遗传距离相对短.
ISSR Analysis of Genetic Diversity of Ancient Tea Plant Resources in Guilin
[Objective]ISSR molecular marker technology was applied to study the genetic diversity and genetic relationship of 50 ancient tea resources in Guilin,in order to fully understand their genetic characteristics,and provide reference for the evaluation and identification of tea germplasm resources,germplasm resources screening,resource protection and utilization in Guilin.[Method]The genetic diversity of 50 ancient tea plant resources and 12 improved variety resources in Guilin was analyzed by 13 primers.[Result]Thirteen primers amplified 223 bands,210 bands were polymorphic,with a polymorphism ratio of 94.17%,indicating that Guilin ancient tea trees have rich genetic diversity.[Conclusion]Guilin ancient tree tea is mostly concentrated in Longsheng County-Ziyuan County-Xing'an County.There is a phenomenon of gene exchange and the genetic distance is relatively short.

Guilinancient tea treegenetic diversityISSR analysis

王小云、谭少波、刘诗诗

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广西壮族自治区茶叶科学研究所,广西 桂林 541004

桂林 古茶树 遗传多样性 ISSR分析

桂林市技术应用与推广计划广西壮族自治区科技重大专项桂林市创新平台和人才计划广西特色作物试验站项目现代农业产业技术体系建设项目广西茶叶创新团队桂林综合试验站项目

20210224-4桂科AA20302018-120220124-16TS202126nycetxgxcxtd-18-04

2023

广西农学报
广西农业广播电视学校

广西农学报

影响因子:0.322
ISSN:1003-4374
年,卷(期):2023.38(6)
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