摘要
基于网络药理学分析桑叶调控免疫功能的作用机制.依托TCMSP数据库获得桑叶有效活性成分和相应作用靶点,构建有效成分——靶点网络图.结合Genecards、OMIM等数据库筛选桑叶免疫调节交集基因,利用STRING数据库构建蛋白相互作用网络(PPI),并对核心靶点进行GO功能富集和KEGG通络分析.研究结果显示,桑叶的主要成分槲皮素、山柰酚、花生四烯酸、β-谷甾醇、四甲氧基木犀草素和豆甾醇等作用于TP53、AKT1、TNF、ESR1、IL6等关键靶点,参与癌症、糖尿病并发症AGE-RAGE信号通路、TNF信号通路、IL-17信号通路等,发挥了免疫调节作用.桑叶具有多成分、多靶点及多途径的特点,对免疫调节起到了良好的作用,为桑叶在畜禽养殖中的应用提供依据.
Abstract
The mechanism of immune function regulation of Folium Mori was analyzed based on network pharmacology.The active components and corresponding target of Folium Mori were obtained based on TCMSP database,and a network diagram of the active components and target was constructed.The immunoregulatory intersection genes of Folium Mori were screened using Genecards,OMIM databases etc,and the protein interaction network(PPI)was constructed using the STRING database.The core target was enriched by GO function and analyzed by KEGG.The results showed that the active components of quercetin,kaempferol,arachidonic acid,β-sitosterol,tetramethoxylluteolin,stigmasterol,etc,in Folium Mori acted on key targets such as TP53,AKT1,TNF,ESR1,IL6,and so on,participating in signal pathways of cancer,AGE-RAGE in cancer and diabetes complications,TNF,and IL-17,and playing an immunomodulatory role.Folium Mori has the characteristics of multi-component,multi-target and multi-pathway,and has shown its effectiveness in immune regulation.The study provided a basis for the application of Folium Mori in livestock and poultry breeding.
基金项目
广西科技重大专项(桂科AA18118041)
广西自然科学基金项目(2023GXNSFBA026204)
2022年、2023年广西农业科技自筹经费项目(Z2022118)
2022年、2023年广西农业科技自筹经费项目(Z2023099)
NETL
NSTL
万方数据