Evaluation of the Inactivation Effect of Alkaline Cleanser on PEDV
In recent years,porcine viral diarrhea has become more and more serious in China,causing a large number of diarrhea cases and death sin piglets,resulting in significant economic losses for the pig industry.In this study,the efficacy of alkaline detergent against porcine epidemic diarrhea virus(Porcine epidemic diarrhea virus,PEDV)was evaluated in order to providea a reference for the selection of suitable cleaning disinfectants in pig farms.The effects of different concentrations of alkaline cleaners A,B,and NaOH on the proliferation activity of Vero cells and the killing effect of PEDV under the interference of organic matter were determined by pH determination,cell proliferation and cytotoxicity tests and cytopathic observation.The results showed that the highest non-toxic concentration of NaOH on Vero cell proliferation activity was 0.03125％,while for alkaline cleaners A and B,it was 2％and 0.5％respectively,indicating their safety superiority over NaOH.At different serum concentration,alkaline cleaner A and B concentrations of 0.5％were found to produce a virus killing effect.This study proved that alkaline cleaner agents have a certain killing effect on PEDV in vitro,which can be used to clean and eliminate the virus in pig farms,helping farms to prevent and control epidemics.
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