Karyotypes and Chromosomal Mapping of Major Ribosomal rRNA Genes in the Leaf Oyster （Dendostrea folium）
Karyotype and chromosomal location of the major ribosomal RNA genes were studied in the leaf oyster （Dendostrea folium） using fluorescence in situ hybridization （FISH）.Metaphase chromosomes were obtained from gill tissue of adult oysters.Probes were prepared by PCR amplification from the internal transcribed spacers （ITS,containing the 5.8S rRNA genes） between the 18S and 28S ribosomal RNA genes,and labeled by PCR incorporation of Biotin-11-dUTP.The results showed that the leaf oyster （D.folium） had a haploid number of 10 chromosomes with a karyotype of ten metacentric chromosomes （10 M）,which was similar to most species in genus Crassostrea.FISH with the ITS probes generated two signals per metaphase,which were hybridized to the telomere regions of the short arms of chromosome 2.This study is the first report of karyotype and chromosomal assignment of the major RNA genes in D.folium.
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