Regulation of Ca~（2＋） Regulators on the CaM Gene Expression during Ethylene-induced Flowering in Guzmania ＇Amaranth＇
Bromeliad flowering induction of ethylene was begun to be applied from the 1930s,it has been a significant effects in the production,however,the mechanism of bromeliad flowering induction of ethylene is not yet clear.In view of the regulation roles of Ca2＋-CaM during in the flowering process of a variety of plants,in order to study the functions of the Ca2＋-CaM system in the ethylene-induced flowering of bromeliad,we employed adult plant of Guzmania＇Amaranth＇ as testing material to be treated by ethylene combining with Ca2＋ accelerator,Ca2＋ chelator or Ca2＋ inhibitor,respectively,the variations of CaM gene expressing characteristics were studied by use of RT-PCR and Northern analysis.The results showed that adding Ca2＋ accelerator accompanying with the ethylene treatment can advance the time of the CaM expressing peak occurring 6 hours instead of 24 hours,whereas adding Ca2＋ chelator or Ca2＋ inhibitor can delay the time of the CaM expressing peak to 48 hours later,respectively.The results were consistent with the process of the corresponding flowering time,which the time of floral organs appeared ahead of about 5 days by adding Ca2＋ accelerator as well as delayed in different extent by the added Ca2＋ chelator or Ca2＋ inhibitor.This study indicated that the Ca2＋ regulators should have some impacts on the regulation of CaM expressing characteristics leading to influence on ethylene-induced flowering of bromeliad.
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