Differential Gene Expression of Rat Testicle＇s Tissues in the Age of 6 and 10 Days
In this research,differential analysis of genome-wide expression of the rat testis tissues in the age of 6 and 10 days was conducted by gene chip technology based on the 12×135K genome-wide EST microarray of rat produced by Roche-NimbleGen.The results showed that there were 4 298 genes with 2-fold or more differentially expressed in the rat testis tissues between the ages of 6 and 10 days,thereinto,1 878 genes were up-regulated expression and 2 420 genes were down-regulated expression.3 154 genes of these differentially expressed genes have been annotated with gene ontology,participating in the 154 biological pathways.Further analysis showed that there were 13 genes with more than 8 fold differential expression,those genes involved in gene ontology categories,such as biological processes,cellular components and molecular functions and so on.Three differentially expressed genes,LOC686076,Cxcl6 and Trib3,were chosen to detect by real-time quantitative RT-PCR,the results exhibited a tread toward the microarray data.Therefore,we preliminary thought that a large number of genes should be involved in the occurrence and proliferation of spermatogonial stem cell in early stages of growth and development of the rat,it might be a synchronizing process of multi-gene expression.
Rat （Rattus norvegicus）Testis tissuesSpermatogonial stem cells （SSCs）Differential expressionGene chip
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