Detection of Hypovirus Protein p29 from Mitochondria of the Chestnut Blight Fungus
p29 is a papain-like protease encoded by hypovirus CHV1-EP713.Previous studies have revealed that expression of p29 in its host,the chestnut blight fungus （Cryphonectria parasitica）,results in reduced virulence and suppression of pigment production and asexual sporulation.Except for co-separation with membrane structures originated from the Golgi apparatus,little was known about the subcellular localization of p29 in the cell.With the success in generating antibody specific to p29 and isolation of high quality mitochondria,p29 was detected from the mitochondria of hypovirus-infected C.parasitica by Western Blot analysis.This finding implies that p29 may be involved in perturbation of mitochondrial function of the host fungus.
Chestnut blight fungus （Cryphonectria parasitica）HypovirusMitochondriaLocalization of p29
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