Identification of Fungus Pathogen by ITS Sequencing：A Judicial Case Study
In this study we chose one judicial case of suspected injury to the pine by air pollutants as a case study,we made an on-site investigation and preliminary morphological identification prior to molecular identifying the pathogen that tentatively caused harm to the pine by air pollutants.The results seemed to be unsupported the charge of the injured pines by air pollutants based on the on-site survey in the pine field and the surrounding areas.In order to figure out reasons for the dead pine trees,we sampled the tentative disease pathogens from the branches of the pines to be observed under the microscope as well as to be incubated for observation of cultured characters.Based on the microscopic morphology of spores and cultured characters of the pathogens,we did preliminary determine the curse leading to dead pines that might be the Pestalotiopsis fungus.To further confirm the pathogenic species,we employed the techniques of molecular biology,the ITS molecular identification,to determine the pathogen by sequencing,aligning and phylogenetic analyzing.The results showed that the pathogen resulting in dead pines should be the fungus Pestalotiopsis vismiae.In this case study,we conducted the phylogenetic analysis of fungal ITS sequences to have rapidly and accurately identified the actual pathogen leading to dead pine trees,which would be the case to provide a new means for identification of forensic evidence in future.
Pine tree （Pinus spp）Fungus pathogenPestalotiopsis vismiaeInternal transcribed spacer （ITS）Case studyJudicial identification
NETL
NSTL
万方数据
