Cloning and Analysis of Promoter-like Sequence of ARAhPR10 Gene from Peanut （Arachis hypogaea L.）
PR10 （pathogenesis-related class10 protein） is considered as a kind of proteins that close associated with the defense of plant diseases and system acquired resistance （SAR）.The 256 bp promoter-like sequence of 5＇ upstream of initial codon of ARAhPR10 gene from Aspergillus flavus-resistant Arachis hypogaea L.cv.Yueyou20 was cloned by using PCR-based genomic DNA walking approach.The prediction analysis of this promoter-like sequence was carried out based on plant cis-acting element database,PLACE.The results showed that the promoter-like sequence contained conservative structural elements of four TATA boxs and two CAAT boxes,and the promoter response element motifs of six W-boxs,one site of BIHD1 and three sites of GT-1,where W-box usually involved in the PR protein response of promoter region of PR proteins.Our preliminary results indicated that the cloned sequences of this study might be the promoter of ARAhPR10 gene.
Peanut （Arachis hypogaea L.）ARAhPR10PromoterCis-acting element
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