Studies on Activities of Extracellular Enzymes in Liquid Culture of Trametes pubescens
In this study,we employed the fungal strain Trametes pubescens Cui7571 as experimental material to analyze the activities of extracellular enzymes in liquid culture,as well as to measure the mycelial biomass and pH values in liquid culture.The results demonstrated that the activities of extracellular enzymes were correlated with the mycelium growth.The increase of mycelial biomass presented S-like curve,which showed logarithmic increase of mycelial growth at the 6th to 8th days after inoculation then reached its peak on the 12th day.The growth peaks for laccase,manganese peroxidase,amylase,carboxymethylcellulose enzyme,pectinase and protease were all observed during the incubation stage.The variations of enzyme activities indicated that the strain （T.pubescens） decomposed lignin firstly,and then used starch and cellulose as carbon sources,protein as nitrogen source.In order to achieve the maximum mycelial biomass and shorten the culture time,it is necessary to provide the sufficient and balancing carbon and nitrogen sources in liquid fermentation.This study indicated that the secreted peaks of different enzymes might be used as criteria of judging the status of mycelial nutrition utilization and fermentation periods to harvest the maximum mycelial biomass for further large scale production.
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