Directed Protein Evolution and Its Applications to Metabolic Regulation in Microbes
Directed protein evolution is an effective and powerful irrational approach for protein engineering.The use of directed protein evolution technology can change the protein metabolic flow,expand or build a new meta-bolic pathway as well as weaken or eliminate unnecessary or harmful metabolites so as to meet the purposes of increasing the yield of metabolites or degrading harmful substances.The technology of directed protein evolution applied in the metabolic regulations would effectively broaden the ranges of metabolic engineering applications.In this review we introduced the main methods of directed protein evolution such as error-prone PCR,DNA shuffling,Stagger Extension Process （StEP）,Random Chimeragenesis on Transient Templates （RACHITT） and so on.And also we reviewed the applications of directed protein evolution in metabolic regulation to modify the key proteins for improving its metabolic ability and metabolite properties.
ProteinDirected protein evolutionMetabolic regulationMicrobes
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