摘要
大泰竹(Thyrsostachys oliveri)是一种在东亚地区广泛分布的竹子,具有重要的生态和经济价值.为获取大泰竹的叶绿体分子生物学特性及明确其系统发育位置,本研究采用高通量测序技术对其叶绿体基因组进行了测序和组装,获得了完整的大泰竹叶绿体基因组序列,并对其进行基因组特征解析和系统发育分析.结果表明:(1)大泰竹的叶绿体基因组为一个典型的双环状结构,全长为139,454 bp,LSC区域的长度为82,986 bp,SSC区域的长度为12,878 bp,IR区域的长度为21,795 bp,全基因组的GC含量为38.90%,共编码了131个基因;(2)共检测到123个SSR位点,其中单核苷酸SSR最多,为102个,占总数的82.93%;(3)系统发育分析表明,大泰竹为禾本科竹亚科中的一个明确分支,与牡竹属(Dendrocalamus)的麻竹(D.latiflorus)和大叶慈(D.farinosus)亲缘关系较近.研究结果明确了大泰竹在竹类植物中的系统发育位置,同时可为竹类分类和鉴定提供新的分子依据.
Abstract
Thyrsostachys oliveri is a bamboo widely distributed in East Asia,with important ecological and economic value.To deepen our comprehension of the molecular biology and evolu-tionary placement of T.oliveri,the research employed advanced high-throughput sequencing tech-niques to meticulously sequence and piece together the chloroplast genome.This effort culminated in the acquisition of a fully intact chloroplast genome sequence for T.oliveri,which was then sub-jected to an exhaustive analysis of its genomic features and phylogenetic analysis.The results showed that:(1)The chloroplast genome of T.oliveri is a typical double ring structure,with a total length of 139454 bp,an LSC region length of 82986 bp,an SSC region length of 12878 bp,and an IR region length of 21795 bp.The GC content of the entire genome is 38.90%,encoding a total of 131 genes;(2)A total of 123 SSRs were detected,with a maximum of 102 single nucleotide SSRs,accounting for approximately 82.93%;(3)The phylogenetic tree shows that T.oliveri belongs to a clear branch of the Bambusoideae in the Poaceae,and is closely related to Dendrocalambus latiflo-rus and D.farinosus.The research results were of great significance for understanding the phyloge-netic of T.oliveri in bamboo plants and provided new basis for bamboo identification.
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