Chloroplast Whole Genome and Phylogenetic Analysis of Thyrsostachys oliveri
Thyrsostachys oliveri is a bamboo widely distributed in East Asia,with important ecological and economic value.To deepen our comprehension of the molecular biology and evolu-tionary placement of T.oliveri,the research employed advanced high-throughput sequencing tech-niques to meticulously sequence and piece together the chloroplast genome.This effort culminated in the acquisition of a fully intact chloroplast genome sequence for T.oliveri,which was then sub-jected to an exhaustive analysis of its genomic features and phylogenetic analysis.The results showed that:(1)The chloroplast genome of T.oliveri is a typical double ring structure,with a total length of 139454 bp,an LSC region length of 82986 bp,an SSC region length of 12878 bp,and an IR region length of 21795 bp.The GC content of the entire genome is 38.90%,encoding a total of 131 genes;(2)A total of 123 SSRs were detected,with a maximum of 102 single nucleotide SSRs,accounting for approximately 82.93%;(3)The phylogenetic tree shows that T.oliveri belongs to a clear branch of the Bambusoideae in the Poaceae,and is closely related to Dendrocalambus latiflo-rus and D.farinosus.The research results were of great significance for understanding the phyloge-netic of T.oliveri in bamboo plants and provided new basis for bamboo identification.
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