Objective To analyze the pathogenic surveillance results of hand,foot and mouth disease in Liuzhou from 2013 to 2019,understand the pathogenic composition of hand,foot and mouth disease in the region and the molecular epidemiological characteristics of EV71,and to provide scientific basis for the prevention and control of local hand,foot and mouth disease.Methods Real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the etiology of the collected hand,foot and mouth disease case specimens.The pathogens of severe or fatal cases were tested for typing,and specimens from 5 EV71-positive hand,foot and mouth disease severe or fatal cases in 2017 were selected for VP1 gene sequence determination and characteristic analysis.Results From 2013 to 2019,a total of 6 509 samples of hand,foot and mouth cases were tested in Liuzhou.There were 4 210 universal enterovirus-positive specimens(69.73％),including 659 samples(15.65％)were positive for EV71,1 159(27.53％)samples were positive for coxsackievirus A16(Cox A16),and 2 392 samples(56.82％)were positive for other enteroviruses.In 2013,2015,2017,and 2019,the main pathogens were other enteroviruses(＞50％),the main pathogens were EV71 and other enteroviruses in 2014,and Cox A16 and other enteroviruses were dominant in 2016 and 2018.The etiological classification of severe or fatal cases from 2014 to 2019 showed that the main pathogens of severe or fatal cases were EV71,Cox A16,Coxsackievirus A6(Cox A6),and Coxsackievirus A2(Cox A2).The pathogenic gene subtypes of the five EV71 strains isolated from severe or fatal cases of hand,foot and mouth disease in the city in 2017 were all C4a subtypes,and the nucleotide homology between the strains'sequences was 95.20％to 100.00％.The amino acid homology was 97.50％to 100.00％.Conclusions The dominant pathogens of hand,foot and mouth disease in Liuzhou has changed from 2013 to 2019.The main pathogen has gradually shifted from EV71 to Cox A16 and other enteroviruses.The pathogens causing severe cases were mainly EV71,Cox A16,Cox A6 and Cox A2,and the dominant genotype of EV71 was C4a subtype.Pathogen surveillance and related molecular epidemiological studies should be further strengthened in the future to provide a basis for the implementation of comprehensive prevention and control measures of hand,foot and mouth disease.
hand,foot and mouth diseaseetiological monitoring resultsEV71VP1 gene
维普
万方数据
