Objective To understand the status and influencing factors of HIV infection in spouses of HIV/AIDS patients in Laibin city,and to provide scientific basis for promoting and improving spouse testing and controlling HIV transmission between spouses in future local AIDS follow-up management.Methods The spouses of HIV/AIDS patients who reported from 2019 to 2020,with current address at Laibin city,could be followed up,married,and had a spouse were included in the monitoring targets.Their HIV antibody detection status were investigated,and univariate and multivariable logistic regression models were used to analyze the influencing factors of HIV infection in the spouses of HIV/AIDS patients.Results A total of 411 spouses of HIV/AIDS patients were investigated,and the positive rate of HIV antibody was 52.31%.Most of them were female,accounting for(63.99%).The majority were over 60 years old or above(46.47%).The education level was mainly primary school(46.96%),and the ethnic group was mainly Zhuang(74.70%).The results of univariate analysis showed that there was a statistical associations between HIV infection in the spouse of HIV/AIDS patients and gender,age,knowledge of access to free counseling and testing,knowledge of the need for regular HIV testing,and knowledge that early treatment can improve quality of life(P<0.05).Multivariable logistic regression analysis showed the risk of HIV infection in the spouse of HIV/AIDS patients:females were 0.362 times(95%CI:0.226-0.580)than males,20-39 years old and 40-59 years old were 0.293 times(95%CI:0.122-0.701)and 0.275 times(95%CI:0.169-0.450)than those over 60 years old,respectively.Lack knowledge of access to free counseling and testing was 11.372 times higher than those who acknowledge(95%CI:3.484-37.118),and lack knowledge of early treatment can improve the quality of life was 28.380 times higher than those acknowledge(95%CI:8.098-99.455).Conclusions The transmission between spouses in Laibin city is one of the important ways of heterosexual transmission of AIDS,screening should be expanded for key populations.Providing counseling and testing for spouses.Early detection,early intervention,early treatment,and reduce HIV transmission between spouses.