Objective To conduct pathogen detection and traceability analysis on a suspected foodborne disease outbreak in a factory in the Tongchuan,Shaanxi,provide references for the development of relevant pathogen detection work in the future.Methods Fecal samples from employees with digestive symptoms,suspicious food samples,and samples of the kitchen environment in the factory were collected.Pathogen types,virulence genes,sources,and drug resistance phenotypes of pathogenic bacteria were detected and analyzed by pathogen culture and isolation,multiple PCR detections,pulsed field gel electrophoresis(PFGE),antimicrobial susceptibility test,and other methods.Results 6 samples of suspicious food residues were collected from the staff canteen in factory,10 samples of the kitchen environment,and 14 samples of patient feces were collected from the hospital.11 strains of(enteroaggregative Escherichia coli,EAEC)were isolated from a total of 30 samples.Among them,9 strains were detected in 14 patient fecal samples.1 strain was detected in 6 kitchen environment samples,and the source was meat chopping boards.1 strain was detected in 6 suspicious food leftover samples,and the source was cold shredded pork.Among the 11 EAEC strains,10 strains carried the virulence genes astA and aggR,and 1 strain carried the virulence gene astA.The PFGE model showed two band types.The 10 strains isolated from the patient's feces samples and the cold shredded pork from the suspicious food residue sample showed the same band type,and a similarity of 100.0％.The band pattern similarity between a strain isolated from the meat chopping board of the kitchen environment sample and other strains was 68.6％.Antimicrobial susceptibility test showed that the 10 EAEC strains isolated from the patient's feces and suspected food leftover samples of cold shredded pork were resistant to ampicillin,piperacillin/tazobactam,ciprofloxacin,sulfamethoxazole,and cefazolin,and the extended-spectrum beta-lactamase(ESBL)phenotype was positive.Conclusions This food-borne illness outbreak was caused by food contaminated with EAEC,The pathogenic strain of this infection carries the virulence genes astA and aggR,is ESBL positive,and is resistant to multiple antibiotics.It is recommended that the health supervision department strengthen food safety management in the future and implement targeted control measures to prevent and control the occurrence of foodborne illness.Meanwhile,it is recommended to strengthen drug resistance surveillance of food-borne pathogens and to improve the ability to provide early warning of multi-drug-resistant bacterial outbreaks.
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