Objective Epidemiological investigation,etiological detection,and traceability analysis were carried out on a food poisoning event suspected to be caused by Staphylococcus aureus.Methods The pathogens were isolated and identified from 4 patient biological specimens and 3 suspicious food samples according to"Diagnostic Criteria for Infections Diarrhea"(WS 271-2007),"National Food Safety Standards-Microbiological examination of food hygiene-Examination of Staphylococcus aureus"(GB 4789.10-2016),and"Diagnostic Standards and Principles of Treatment for Staphylococcus Food Poisoning"(WS/T 80-1996).Detection of the total enterotoxin and enterotoxin type of Staphylococcus aureus isolated strains.The homology of strains was analyzed by pulsed field gel electrophoresis(PFGE).Results A total of 4 cases were found(all confirmed cases),with clinical manifestations mainly including vomiting(100％),diarrhea(100％),and abdominal pain(75％).Five strains of Staphylococcus aureus were detected from 4 patient biological specimens and 1 suspicious food sample(hot dog bread).The five strains were tested and all produced three types of enterotoxin:SEA,SED,and SEE.PFGE molecular typing results showed that the band pattern similarity of the five strains was 100％and they came from the same source.Conclusions This food poisoning incident was caused by Staphylococcus aureus producing SEA,SED,and SEE contaminating hot dog buns.In the future,schools should strengthen food safety education,and relevant government departments should increase supervision of food production enterprises.Food production enterprises should actively carry out hygiene knowledge training for managers and employees,enhance their hygiene awareness,and avoid the occurrence of food poisoning incidents.
food poisoningStaphylococcus aureusPulse field gel electrophoresis
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