Objective To understand the current status of Clonorchis sinensis infection in cats and dogs in Quanzhou county,Guangxi,and to further explore whether clonorchiasis is endemic in the narrowly defined non-endemic areas,providing a basis for comprehensive prevention and control of the disease.Methods A stratified random sampling method was employed to select one farmer's market from each of the five directions(east,south,west,north,and central)of Quanzhou county to conduct surveys on a quarterly basis.A total of 107 cats and 84 dogs were sampled.Complete livers and gallbladders of cats and dogs were collected,and liver lesions were observed and recorded.Suspected worms were also collected for identification,measurement,classification,and calculation of infection rates.COX1 gene amplification and sequencing were performed on the collected worms with different morphologies,and the species of the worms were determined through BLAST comparison and analysis.Chi-square test and t-test were used to analyze the influencing factors of Clonorchis sinensis infection.Results In this survey,41.12％of cats and 57.14％of dogs were sourced locally,and the rest came from other places(Hubei).The livers and gallbladders of 107 cats and 84 dogs were examined.The infection rate of Clonorchis sinensis in cats was 61.68％(66/107),with a maximum of 1 070 worms per individual cat.The infection rate in dogs was 2.38％(2/84),which were locally infected.There was a statistically significant difference in the distribution of worms between cats and dogs with different infection levels(x2=47.353,P＜0.001);as the infection level increased,the probability of ectopic parasitism in the gallbladder also increased.There were statistically significant differences in the infection rates between cats and dogs with gross pathological changes(such as yellowing of the liver surface or thickening and dilation of the liver bile duct wall)and those without gross pathological changes(x2cat=49.509,Pcat＜0.00l;Pdog＜0.001).Significant differences were also observed in liver weight(g)between the positive and negative groups of Clonorchis sinensis in cats and dogs(tcat=3.537,Pcat＜0.01;tdog=4.185,Pdog＜0.001).Three different sources of worms were isolated from local cats,dogs,and cats from Hubei,and their size and testicles were quite different.Morphological and COX1 gene identification confirmed that they were all Clonorchis sinensis.Conclusions Although Quanzhou county in Guangxi is considered a narrowly defined non-endemic area for clonorchiasis,Clonorchis sinensis can be detected in cats and dogs sold in the market,particularly with a high infection rate in cats.Therefore,further investigation and prevention and control of clonorchiasis in animals in non-endemic areas should be further strengthened.
Clonorchis sinensisinfluencing factor analysisCOX1 genenon-endemic areas in the narrow sense
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