Objective To explore the contamination of Vibrio parahaemolyticus in aquatic products sold in Ganzhou,analyze its virulence genes and molecular typing characteristics,and provide a scientific basis for the outbreak,traceability and food safety monitoring of foodborne diseases caused by Vibrio parahaemolyticus in the region.Methods 95 samples of aquatic products sold in various farmer's markets and supermarkets in Ganzhou from 2022 to 2023 were collected for strain isolation and identification.The distribution of three virulence genes,tdh,trh,and tlh,in the isolated strains was screened by triple real-time fluorescent polymerase chain reaction,and homology was analyzed by pulse field gel electrophoresis(PFGE).Results A total of 49 Vibrio parahaemolyticus strains were isolated from 95 aquatic products,with a positive rate of 51.58％,including 90.63％crustaceans,28.89％freshwater fish frogs,and 38.89％bivalves.The positive rate of tlh gene detected in 49 strains of Vibrio parahaemolyticus was 100.00％,and the positive rate of trh gene detected was 4.08％,with no tdh gene.For 49 Vibrio parahaemolyticus strains,except for three strains that were degraded,the analysis results of 46 strains were visible.A total of 43 different Electrophoresis bands were found,and the similarity between bands was 53.60％-100.00％.A total of 26 clones,with widely distributed restriction enzyme band patterns.Conclusions From 2022 to 2023,Vibrio parahaemolyticus contamination in commercially available aquatic products in Ganzhou is relatively serious,but the virulence genes carrying rate is low.Homologous strains are found,but there is no significant dominant cluster,and the strains have rich genetic diversity,suggesting that they have different origins.
aquatic productsVibrio parahaemolyticusvirulence genesPulsed-field gel electrophoresis(PFGE)molecular typing
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