Gastroprotective Effect of Corydalis Hendersonii on Ethanolic Gastric Ulcer in Mice Via NF-κB Signaling Pathway
Objective The model of ethanolic gastric ulcer in mice was established by gavage of anhydrous etha-nol,and the protective effect of the total extract of Corydalis nepalensis on ethanolic gastric ulcer in mice was studied.Methods Sixty KM mice were randomly divided into six groups:blank control group,model group,omeprazole-positive group,and low-dose,medium-dose and high-dose groups of daily Guanzima total extract,with 10 mice in each group.After one week of gavage administration,fasting without water for 24 hours,and 1.5 hours after the last administration,except for the blank control group receiving the same volume of physiologi-cal saline,the other five groups were given 0.1 mL/10g anhydrous ethanol by gavage according to body weight for 1 hour to establish a mouse ethanol induced gastric ulcer model.After 1 hour of modeling,blood was taken from the stomach to calculate the incidence and inhibition rate of ethanol induced gastric ulcer injury.Serum su-pernatant and gastric tissue homogenate were prepared,and hematoxylin eosin(HE)staining was used to observe pathological changes in gastric tissue;The reagent kit detects the levels of nitric oxide(NO),superoxide dis-mutase(SOD)in serum,and malondialdehyde(MDA)and myeloperoxidase(MPO)in gastric tissue.The mRNA expression of p65,IKK-β,TNF-α and IL-1β in gastric tissues was detected by quantitative fluorescence PCR(qRT PCR).Results The gastric mucosa of mice in the normal control group was smooth and intact without hem-orrhage,while the mucosal hemorrhage and tissue necrosis of different degrees were observed in the remaining mice in all groups.The mice in the model group had the most severe gastric injury,which showed a large bleed-ing area with bright red color.Compared with the normal group,the HE staining map of the model control group showed abnormal overall tissue structure,small necrosis in the mucosal layer,loose arrangement of connective tissue in the submucosal layer,rare infiltration of lymphocytes,significantly higher mRNA expression levels of NO in serum,MDA and MPO in gastric tissues and p65,IKK-β,TNF-α and IL-1β in gastric tissues(P<0.05),serum Compared with the model group,the positive group and the group administered with high,medium and low doses of Corydalis nepalensis total extract showed punctate or striated bleeding,a significant reduction in the ar-ea of bleeding,a significant decrease in the incidence of injury(P<0.05),a significant improvement in the histo-pathological damage in the stomach of mice,and different degrees of improvement in serum and tissue-related indexes.Conlusion The total extract of Corydalis nepalensis can inhibit the mRNA expression of key genes p65,IKK-β,TNF-α and IL-1β in NF-κB signaling pathway and suppress oxidative stress and inflammatory re-sponse,which can have some protective effects on ethanolic gastric ulcer in mice.
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NSTL
万方数据
维普
