Analysis on the Root Endophytic Bacteria of Rheum Lhasaense and Rhizosphere Soil Microbial Diversity based on High-throughput Sequencing
The endophytes and rhizosphere soil microorganisms in root of Rheum lhasaense were analyzed using high-throughput sequencing technology,bioinformatics,and statistical analysis to explore the diversity of endo-phytes and rhizosphere soil micro-organism.The results of clustering annotation indicated that there is a total of 90 shared fungal operational taxonomic units(OTU)in the roots and rhizosphere soil,with 452 unique OTU in the roots and 635 unique OTU in the rhizosphere soil.Additionally,there were 622 shared bacterial OTU in the roots and rhizosphere soil,with 982 unique OTU in the roots and 5 618 unique OTU in the rhizosphere soil.Based on α diversity analysis,the number of species,species richness and diversity of rhizosphere soil bacteria in Rheum lhasaense were significantly higher than those in root endophytes(P<0.05).The number of fungi spe-cies in rhizosphere soil was larger than that of root endophytic fungi,however,there was no significant difference in community diversity between rhizosphere soil fungi and endophytic fungi(P>0.05).β diversity analysis indi-cated that there is significant difference in the microbial community structure between endophyte and rhizo-sphere soil microorganisms in Rheum lhasaense(q<0.05).Ascomycota was identified as the dominant phylum of the fungal community in both roots and rhizosphere soil samples.Bacteroidota had the highest relative abun-dance in endophytic bacterial community,with a relative abundance of 75.44%.The dominant phylum in rhizo-sphere soil is Proteobacteria with a relative abundance of 44.36%.At the genus level within roots samples Peni-cillium exhibited the highest relative abundance of 21.8%,whereas Truncatella had a relative abundance of 26.16%within rhizosphere soils samples.JC017 has the highest relative abundance in roots that can be classi-fied in bacterial communities,with a relative abundance of 73.44%.while Bacillus_BD had a relative abundance of 6.7%in rhizosphere soils.
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