首页|青海草地早熟禾全长转录组测序及功能分析

青海草地早熟禾全长转录组测序及功能分析

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青海草地早熟禾(Poa pratensis L.cv.Qinghai)是一种优质的多年生冷季生态草,被广泛应用于生态修复、城市绿化等领域.该研究利用PacBio Iso-Seq测序技术,对青海草地早熟禾进行了全长转录组测序,并对其进行了结构分析和功能注释.研究共获得37 613条全长转录本,其中95.24%的转录本在NR、GO、KEGG等数据库中得到了注释.功能注释结果显示,转录本主要与新陈代谢、信号传导等通路相关.此外,研究还预测了8 750个转录因子,为进一步解析青海草地早熟禾产量形成机制提供了重要资源.
Full-length Transcriptome Sequencing and Functional Analysis of Poa Pratensis L.cv.Qinghai
Poa pratensis L.cv.Qinghai is a high-quality perennial cool-season ecological grass that has been widely used in ecological restoration,urban greening and other fields.In this study,the full-length transcriptome sequencing was performed on Poa pratensis and structural analysis and functional annotation was conducted on it by bioinformatics analysis.A total of 37 613 full-length transcripts were obtained,of which 95.24%was annotat-ed in databases such as NR,GO,KEGG and so on.The results of functional annotation indicated that that the transcript is mainly related to pathways of metabolism and signal transduction.Additionally,8 750 transcription factors were predicted providing important resources for further elucidating the yield formation mechanism of Poa Pratensis L.cv.Qinghai.

Poa Pratensis L.cv.Qinghaifull-length transcriptomethird-generation sequencingfunctional an-notation

慕星宇、刘颖、乔志梅、赵洪奎、鲍根生

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青海大学青海省畜牧兽医科学院青海省高寒草地适应性管理重点实验室 青海 西宁 810006

青海大学畜牧兽医科学院青海省青藏高原优良牧草种质资源利用重点实验室 青海 西宁 810006

青海省果洛州农牧综合服务中心 青海 果洛 814000

青海草地早熟禾 全长转录组 三代测序 功能注释

2024

高原科学研究

高原科学研究

ISSN:
年,卷(期):2024.8(4)