Based on Network Pharmacology,Molecular Docking and Experimental Verification,the Target and Signal Pathway of Sanguo Tang for Preventing and Treating Hyperlipidemia Were Explored
Objective:To investigate the potential active components in San Guo Tang that treat hyperlipidemia and to elucidate the key signaling pathways involved in its lipid-lowering effects.Methods:We employed net-work pharmacology,molecular docking validation,and pathway enrichment analysis to identify the potential ef-fector molecule MAPK involved in the blood sugar-lowering process of San Guo Tang.A hyperlipidemia mouse model was established,and mice were treated with San Guo Tang and MAPK inhibitors.Western blotting was used to assess changes in protein molecules and signaling pathways within the mice.Results:The phosphoryla-tion level of PI3K/AKT signaling pathway in the liver of hyperlipidemic mice increased,and the magnitude of the increase was positively correlated with the blood lipid levels of the mice.San Guo Tang can inhibit the phosphory-lation of the PI3K/AKT signaling pathway by suppressing the expression of MAPK,thereby reducing blood lipid levels.Conclusion:San Guo Tang possesses lipid-lowering efficacy,which is associated with the inhibition of MAPK expression and the reduction of PI3K/AKT signaling pathway phosphorylation.Further research is war-ranted to explore MAPK as a target and signaling pathway for San Guo Tang's lipid-lowering actions,as well as to uncover additional mechanisms and effector molecules involved in its lipid-lowering effects.
San Guo Tanghyperlipidemiatherapeutic targetsPI3K/AKTMAPK
万方数据
维普
