Experimental design of yeast gene editing using CRISPR/Cas9 technology
Combining the practical teaching requirements of bioengineering and the cutting-edge direction of the disci-pline,an experimental project called"Yeast genome editing experiment design using CRISPR/Cas9 technology for bioengi-neering majors"was designed.The project adopts a transient CRISPR/Cas9 technology,which requires no plasmid construc-tion during the experiment,requires less experimental materials,and has strong experimental feasibility.This experimental project helps to strengthen students'understanding of the frontiers of life science and cultivate their spirit of exploration and innovation.
YeastCRISPR/Cas9TransientGene editingFrontiers of disciplines