Combining the practical teaching requirements of bioengineering and the cutting-edge direction of the disci-pline,an experimental project called"Yeast genome editing experiment design using CRISPR/Cas9 technology for bioengi-neering majors"was designed.The project adopts a transient CRISPR/Cas9 technology,which requires no plasmid construc-tion during the experiment,requires less experimental materials,and has strong experimental feasibility.This experimental project helps to strengthen students'understanding of the frontiers of life science and cultivate their spirit of exploration and innovation.
关键词
酵母/CRISPR/Cas9/瞬时/基因编辑/学科前沿
Key words
Yeast/CRISPR/Cas9/Transient/Gene editing/Frontiers of disciplines