Studies on Coniothyrium minitans JN-CM strain in shake flask media and its solid fermentation
In this paper,the viability of Coniothyrium minitans JN-CM in the shake flask media was analyzed and the its solid fermentation conditions for generating spores were optimized. By measuring the rate of the conidium germination and infection,the activity of conidia after shake flask and solid fermentation was proved,and then the conditions for shake flask and solid fermentation were optimized. The results showed that spores could reach 1. 09 × 108 cfu/mL medium and 5. 4123 g/L mycelium at initial pH 7. 0 and shake flask fermentation for 6 days. Under the conditions of 100 μL liquid seed (1 × 107 cfu/mL),5 g bran,substrate material to water ratio 1∶1. 5,pH 5. 5 and fermentation for 11 days,spores could reach 2. 65 × 1010 cfu/g.
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