Cellulase from Bacillus has excellent catalytic properties such as heat and alkali resistance,so it has been attracted wide attention.In this paper,carboxymethyl cellulase(CMCase)was produced with the screened Bacillus sp.B55.On the basis of determining the effect of carbon source,nitrogen source,metal salt,carboxymethyl cellulose addition and inoculation amount on the CMCase production of the strain B55,the fermentation cultural process was further optimized by orthogonal experiment design.The results indicated the optimal medium was 30 g/L glycerine,5 g/L glucose,3 g/L soybean peptone,16 g/L yeast powder,4 g/L CMC,3 g/L K2HPO4,3 g/L NaH2PO4,pH 7.0,and then 401.34 U/mL of the maximum CM Case activity was obtained at 48 h under the conditions of 4%(V/V)inoculums and culture temperature of 32℃,so which had potential for scale production and application.
关键词
羧甲基纤维素酶/芽孢杆菌/培养优化/发酵生产
Key words
Bacillus sp/Carboxymethyl cellulase/culture optimization/fermentation production
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