The aim of this study was to investigate the effects of rose essential oil at different concentrations on D-gal induced skin fibroblast senescence.There were blank control group,D-gal group and D-gal+ rose essential oil group.β-gal staining was used to detect the number of positive cells,EdU-594 staining was used to detect the proliferation of cells and scratch assay was used to detect the migration of cells.The β-gal staining results showed that the number of senescence staining positive cells decreased in the D-gal+ rose essential oil group compared with the control group(D-gal group)(p＜0.001);EdU-594 staining showed that compared with the control group,the number of proliferating cells in D-gal+ rose essential oil group increased(p＜0.01);The results of cell migration experiment showed that compared with the control group,the cell migration rate of D-gal+ rose essential oil group increased(p＜0.001).Therefore,rose essential oil can reduce the number of cell senescence in D-gal model,improve the proliferation ability of skin fibroblasts,and increase the migration ability of skin fibroblasts.
关键词
玫瑰精油/皮肤成纤维细胞/衰老/增殖/迁移
Key words
rose essential oil/skin fibroblasts/aging/proliferation/transfer
维普
万方数据