Preliminary Study on Optimization of Extraction Process and Antioxidant Activity of Total Flavonoids from Artemisia argyi Leaves
This experiment uses orthogonal design to optimize the extraction process of flavonoids from Artemisia argyi leaves and preliminarily explore their antioxidant activity.After experiments,it was found that the optimal extraction process for flavonoids from Artemisia argyi leaves is to soak the powder of Artemisia argyi leaves with a ratio of 1∶25(g/mL)in 80%ethanol solution for 4 hours and extract it with ultrasound for 50 minutes,with an extraction rate of 3.53%.There is a positive correlation between the concentration of Artemisia argyi flavonoid extract and total reducing ability.When the concentration of Artemisia argyi flavonoid is 1 mg/mL,the clearance rates of DPPH·and·OH are 96.35%and 97.49%,indicating strong antioxidant activity.
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