c-di-AMP,as an emerging second messenger molecule,has physiological functions such as global regulation of intracellular osmotic pressure balance,DNA damage,and biofilm formation.Our laboratory initially screened a thermophilic Streptococcus strain S-3 with high extracellular polysaccharide production,but lacked research on its c-di-AMP signaling pathway.This study screened the c-di-AMP synthase(StDAC)from the entire genome of Streptococcus thermophilus S-3 and predicted its secondary structure;The results show that the secondary structure of StDAC contains 42.98%α-Spiral,15.32%β-Folding and 38.30%irregular curling.Using SWISS-MODEL and Modeler for single template and multi template homologous modeling of StDAC,the model established by SWISS-MODEL was validated by Ramachandran,Errat,and Verify-3D evaluation indicates that its structure is relatively reasonable;The model established by Modeller was only evaluated by Ramachandran,with a low overall structural score.The predicted three-dimensional model of Streptococcus thermophilus StDAC in this study lays the foundation for analyzing its structure and function.
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