Optimization of High Density Culture Conditions of Ligilactobacillus salivarius Based on Response Surface Analysis
Ligilactobacillus salivarius has important commercial value.It is very important to realize high density fermentation and preparation for industrial application.In this experiment,L.salivarius AR809 was used as the target strain,and its high-density culture conditions were optimized by single factor experiment and response surface design.The optimum medium was formulated as follows(g/L):glucose 21.111 9,casein peptone 10,beef extract 10,yeast extract 7.047 5,diammonium hydrogen citrate 2.063 1,potassium phosphate dibasic 2,sodium acetate trihydrate 6.3,magnesium sulphate heptahydrate 0.58,manganese sulphate monohydrate 0.25,and tween 80 0.5 mL/L.In conclusion,this paper provides a data base for the next high-density culture of L.salivarius AR809.
万方数据
维普
