Screening of Lactic Acid Bacteria Inhibiting Helicobacter Pylori and Study on the Characteristics of Antibacterial Substances
In order to obtain lactic acid bacteria that can inhibit Helicobacter pylori,this study has screened 10 strains of lactic acid bacteria from pickled vegetables and conducted experiments on inhibition of Helicobacter pylori using the Oxford Cup method on these 10 strains.The results show that three of these 10 strains,PW-2,PW-5,and PW-7,have antibacterial activity against Helicobacter pylori.The PW-2 strain has the largest antibacterial diameter.This strain has been identified as Lactobacillus plantarum through morphological characteristics and 16S rDNA gene sequence alignment analysis.Further research has been conducted on the antibacterial active substances of this strain,and after excluding lactic acid and H2O2,it still shows antibacterial activity.Then,the subsequent protease sensitivity test shows that the antibacterial substances in the fermentation broth of the strain are proteins or peptides.In addition,the antibacterial substances of strain PW-2 exhibit strong antibacterial activity under acidic conditions and have the certain temperature stability.
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