高效液相色谱法测定疫苗菌体破碎液中D-核糖的技术研究
Determination of D-ribose in the Bbroken Liquid of Vaccines by High Performance Liquid Chromatography
黄婷 1徐晓萍 1彭雪琦1
作者信息
- 1. 江苏省生产力促进中心,江苏南京 210042
- 折叠
摘要
文章以示差折光为检测器,建立高效液相色谱法定量测定疫苗菌体破碎液中D-核糖的方法.该方法的测定条件为:色谱柱为安捷伦ZORBAX NH2氨基色谱柱,流动相为0.005 mol/L的稀硫酸溶液,柱温为50 ℃,进样量为20 μL.D-核糖检测方法保留时间为3.684 min,得到的D-核糖的线性回归方程式为y=194.037 72x+10.834 09,相关性为0.999 99.式中,y为D-核糖的质量浓度;x为对应的峰面积.该方法精密度、稳定性和重现性表现良好,D-核糖的回收率范围为90.2％～102％,灵敏度高,能实现目标峰与杂峰的有效分离,因此可被广泛用于疫苗菌体破碎液等样本中D-核糖浓度的测定.
Abstract
A high performance liquid chromatography method for quantitatively determining the D-ribose with refractive index detector(RID)is established.The measurement conditions for this method are as follows:the Agilent ZORBAX NH2 column,0.005 mol/L of diluted sulfuric acid solution as the mobile phase,50℃ column temperature and the 20 μL injection volume.The retention time of D-ribose is 3.684 min.The linear regression equation for obtaining D-ribose is y=194.037 72x+10.834 09,and the correlation is 0.999 99,in which the y means the mass concentration of D-ribose and the x means the corresponding peak area.This method exhibits good precision,stability,and reproducibility.The recovery of D-ribose ranges from 90.2％to 102％.This improved method has high sensitivity and can achieve effective separation of target peak and impurity peak.Therefore,it can be widely used for measuring the D-ribose levels in a variety of the broken liquid of vaccines.
关键词
D-核糖/示差检测器/疫苗菌体破碎液Key words
D-ribose/refractive index detector(RID)/broken liquid of vaccines引用本文复制引用
基金项目
江苏省生产力促进中心青年人才基金项目(D2023003)
出版年
2024
国家科技期刊平台
NSTL
万方数据