Determination of D-ribose in the Bbroken Liquid of Vaccines by High Performance Liquid Chromatography
A high performance liquid chromatography method for quantitatively determining the D-ribose with refractive index detector(RID)is established.The measurement conditions for this method are as follows:the Agilent ZORBAX NH2 column,0.005 mol/L of diluted sulfuric acid solution as the mobile phase,50℃ column temperature and the 20 μL injection volume.The retention time of D-ribose is 3.684 min.The linear regression equation for obtaining D-ribose is y=194.037 72x+10.834 09,and the correlation is 0.999 99,in which the y means the mass concentration of D-ribose and the x means the corresponding peak area.This method exhibits good precision,stability,and reproducibility.The recovery of D-ribose ranges from 90.2％to 102％.This improved method has high sensitivity and can achieve effective separation of target peak and impurity peak.Therefore,it can be widely used for measuring the D-ribose levels in a variety of the broken liquid of vaccines.
D-riboserefractive index detector(RID)broken liquid of vaccines
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