一株新型高效聚磷菌Glutamicibacter sp.G2的分离鉴定及除磷特性
Isolation,Identification and Phosphorus Removal Characteristics of A Novel High-efficiency Phosphorus-accumulating Bacterium Glutamicibacter sp.G2
习彦花 1吴健 1孙立博 2魏帅强 2吕亚天 3程辉彩1
作者信息
- 1. 河北省科学院生物研究所,河北石家庄 050081
- 2. 河北省科学院生物研究所,河北石家庄 050081;河北工程大学园林与生态工程学院,河北邯郸 056038
- 3. 河北省科学院生物研究所,河北石家庄 050081;河北地质大学水资源与环境学院,河北石家庄 050031
- 折叠
摘要
聚磷菌(Phosphorus accumulating bacteria,PAOs)在生物除磷系统(EBPR)的强化过程中发挥着重要作用.挖掘新型高效聚磷菌并优化其除磷工艺,对于废水生物除磷技术的发展具有重大意义.文章通过富集驯化、分离纯化,结合MOPS蓝白斑(10x)初筛、Albert异染颗粒复筛等方法从某污水处理厂污泥样品中筛选出一株高效聚磷细菌G2,将其鉴定为盐生谷氨酸杆菌(Glutamicibacter halophytocola),并通过单因素试验探究pH、温度、接种量、初始磷含量,以及水体中的阳离子浓度对菌株G2生长及除磷的影响.实验结果表明,该菌株在初始磷浓度为30 mg/L、pH为8.0、8.0％接种量、32 ℃的条件下发酵72 h后,磷去除率可达78.79％.同时,添加2.0％的Mg2+可以将菌株G2在人工合成废水中的除磷率提高17.50％.文章所筛选出的盐生谷氨酸杆菌G2在人工合成废水中表现出了良好的除磷效果,有望为废水生物除磷技术提供优质菌种资源.
Abstract
Phosphorus-accumulating bacteria(PAOs)plays an important role in the enhanced biological phosphorus removal(EBPR)system.Exploring new and efficient phosphorus-accumulating bacteria and optimizing the optimal phosphorus removal process are of great significance for the further development of biological phosphorus removal technology in wastewater.This paper uses enrichment and domestication,and separation and purification,combined with MOPS blue and white spots(10x)and initial screening and Albert particle re screening and other methods,to selected a high-efficiency phosphorus-accumulating bacterium G2 from sludge samples of a sewage treatment plant,which is identified as Glutamicibacter halophytocola.The effects of pH,temperature,inoculation amount,initial phosphorus content,and cation concentration in water on the growth and phosphorus removal of strain G2 are investigated through a single factor experiment.The results show that under the initial phosphorus concentration of 30 mg/L,pH 8.0,8.0％inoculation amount,and temperature of 32 ℃,the phosphorus removal rate of the strain can reach 78.79％after 72 hours of fermentation.Meanwhile,2.0％Mg2+can significantly improve the phosphorus removal rate of strain G2 in artificially synthesized wastewater by 17.50％.The Glutamicibacter sp.G2 screened in this paper has a good phosphorus removal effect in artificially synthesized wastewater,and is expected to provide excellent bacterial resources for biological phosphorus removal technology in wastewater.
关键词
聚磷菌/分离鉴定/Glutamicibacter/sp.G2/合成废水/除磷特性Key words
phosphorus-accumulating bacteria/isolation and identification/Glutamicibacter sp.G2/synthetic wastewater/phosphorus removal characteristics引用本文复制引用
基金项目
河北省重点研发项目(22373803D)
河北省"三三三人才工程"资助项目(C20221007)
河北省科学院高层次人才培养与资助项目(2023G08)
出版年
2024
国家科技期刊平台
NSTL
万方数据