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平板计数法检测化妆品中需氧细菌总数的不确定度评估

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文章将不确定度原理引入平板计数中,以测量需氧细菌总数检测过程中平皿计数法的检测误差.检测方法参照USP 2023<61>中的"非无菌产品微生物限度检查",采用微生物计数法定量检测化妆品中的需氧细菌总数,并参照JJF1059.1-2012《测量不确定度评定与表示》中的要求,用需氧细菌总数对数值表示,通过评定需氧细菌总数检测中平皿计数法测量的不确定度,探讨平皿计数的检测误差[1].结果表明,平皿法检测需氧菌总数的测量误差主要由A类评定的不确定度所致,而B类评定不确定度造成的误差可以忽略不计,因而据此得出可以扩展不确定度.研究所得的通过对需氧细菌总数检测中平皿计数结果的测量进行不确定度评定,可用于评估平皿计数法的检测误差[2].
Uncertainty Assessment of Total Number of Aerobic Bacteria in Cosmetics by Plate Counting Method
In this paper,the uncertainty principle was introduced into plate counting to investigate the detection error of plate counting method in the process of detecting the total number of aerobic bacteria.The method is based on USP 2023<61>;Microbial limit check of non-sterile products,microbial counting method for quantitative detection of the total number of aerobic bacteria in cosmetics,according to the requirements of JJF1059.1-2012《Evaluation and Expression of Measurement Uncertainty》,Using the total number of aerobic bacteria to the value of the value,by assessing the measurement uncertainty of the plate counting method in the total number of aerobic bacteria detection.The detection error of plate count[1]is discussed.The results show that the error of the plate method for detecting the total number of aerobic bacteria mainly comes from the uncertainty of class A evaluation,and the error caused by class B evaluation uncertainty can be ignored,So as to obtain the extended uncertainty.Conclusion The measurement uncertainty of the plate count result in the total number of aerobic bacteria detection can be used to evaluate the detection error of the plate count method[2].

CosmeticsPlate counting methodTotal number of aerobic bacteriaUncertaintyStandard uncertaintyExtended uncertainty

张超锋

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南德商品检测(上海)有限公司,上海 201108

化妆品 平板计数法 需氧细菌总数 不确定度 标准不确定度 扩展不确定度

2024

工业微生物
全国工业微生物信息中心 上海市工业微生物研究所

工业微生物

影响因子:0.293
ISSN:1001-6678
年,卷(期):2024.54(3)
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