Determination of Water-soluble Components in Banxia and Qingbanxia
The metod which can analyze 6 kinds of chemical constituents,such as urinary pyrimidine,Xanthine,xanthine,urinary glycosides,inosine and adenosine in Banxia,and the 5 components of Middle xanthine,urinary glycosides,inosine,guanosine and adenosine in Qingbanxia has been established.High Performance liquid chromatography(HPLC)was used to analyze the water-soluble components in Banxia and in Qingbanxia,whose chromatographic column was Elit ODS2(4.6 mm×250 mm,5 μm),flow phase was methanol-0.1％formic acid water,gradient elution,detection wavelength 265 nm.The content of 6 water-soluble components in Banxia and the content of 5 kinds of water-soluble components in the Qingbanxia were calculated by regression equation.The 6 components in Banxia and the 5 components in Qingbanxia showed a good linear relationship.In addition to guanosine,the content of nucleoside components in Qingbanxia was significantly reduced compared with Banxia;the content of guanosine in Qingbanxia increased,and the content of water-soluble components determined in Qingbanxia after concoction was lower than that in Banxia.The content of water-soluble components measured in the finished Qingbanxia was lower than that in the middle half of the Banxia,and the contents of the urine pyrimidine and xanthine in the Banxia were disappeared or converted into other components.This method can detect 6 water-soluble components in Banxia and 5 water-soluble components in Qingbanxia,which is simple,sensitive and controllable,and can determine the content of water-soluble components in Banxia decoction tablets and Qingbanxia.
国家科技期刊平台
NSTL
万方数据
