摘要
文章旨在分析纳木错裸鲤(Gymnocypris namensis)肠道微生物的群落结构和多样性特征,并对其潜在功能进行探索,从而为其健康稳定生长和发育以及保持纳木错生态系统的稳定性提供数据支撑.文章通过Illumina Miseq平台对纳木错裸鲤肠道微生物进行高通量测序,共计得到优化后的序列554 607条,优化总碱基数229 224 641 bp.经DADA2降噪后,8个样本共产生346 272条序列,获得2598个ASVs,统计共得到26个门、63个纲、134个目、189个科、251个属、361个种.雌、雄纳木错裸鲤在 f_Desulfosarcinaceae、f_Rubritaleaceae、f_norank_o_norank_c_Berkelbacteria 这三个菌属之间存在显著差异(P＜0.05).功能预测结果显示,肠道微生物编码的基因与蛋白质加工、合成以及新陈代谢紧密相关,涉及翻译,核糖体结构和生物发生及氨基酸运输和代谢功能类群的相对丰度较高.
Abstract
The purpose of this paper was to analyze the community structure and diversity characteristics of the intestinal microorganisms in Gymnocypris namensis,and to investigate the possible roles of the intestinal microorganisms,so as to provide data supports for the healthy and stable growth,development and stability of Gymnocypris namensis,and the stability of the Namtso ecosystem.This paper used the Illumina Miseq platform for high-throughput sequencing of intestinal microorganisms in Gymnocypris namensis,the total number of optimized sequences was 554 607,and the total number of optimized bases was 229 224 641 bp.After DADA2 denoising,a total of 346 272 sequences were generated from 8 samples,and 2 598 ASVs were obtained.A total of 26 phyla,63 classes,134 orders,189 families,251 genera and 361 species were identified.There is significant difference between female and male Desulfosarcinaceae in f_Rubritaleaceae and f_norank_o_norank_c_Berkelbacteria(P＜0.05).Functional prediction results show that the genes encoded by intestinal microorganisms were mostly related to protein processing,synthesis and metabolism,and the relative abundance of translation,ribosome structure and biogenesis,as well as amino acid transport and metabolic functional groups was relatively high.
基金项目
西藏自治区重点研发项目(XZ202001ZY0036N)
国家科技期刊平台
NSTL
万方数据