不同生长阶段白条双锯鱼肠道微生物菌群结构多样性研究

Study on the Diversity of Intestinal Microflora Structure in Amphiprion Frenatus at Different Growth Stages

朱婷 ¹李涛 ²李恭如 ¹黎双飞¹

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作者信息

1. 深圳大学生命与海洋科学学院,广东深圳 518055
2. 中国水产科学研究院南海水产研究所深圳试验基地,广东深圳 518121
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摘要

文章通过收集人工养殖的3月龄、6月龄、12月龄、18月龄的白条双锯鱼(amphiprion frenatus)肠道样品,分别提取其肠道DNA,经PCR扩增后对16S rDNA的V3～V4区进行高通量测序,分析其肠道微生物菌群结构的组成差异.结果表明,3月龄和18月龄的白条双锯鱼的肠道内OTU数量、Chao1指数、Shannon指数和Simpson指数较其他阶段更高,显示出更加丰富的物种多样性.在门水平上,白条双锯鱼肠道微生物的主要优势菌门为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和梭杆菌门(Fusobacteria);在属水平上,其主要优势菌属包括发光菌属(Photobacterium)、假单胞属(Pseuomonas)、无色杆菌属(Achromobacter).文章的研究结果给观赏鱼养殖饵料筛选和益生菌试剂研发提供了一定理论支撑.

Abstract

Intestinal tract samples of artificially farmed 3-month-old,6-month-old,12-month-old and 18-month-old Amphiprion frenatus were collected in this research.Then,their intestinal DNA was extracted,the V3-V4 region of 16S rDNA was amplified by PCR for high-throughput sequencing,and the differences in the composition of intestinal microbial community structure were analyzed.The results showed that the number of OTU,Chao1 index,Shannon index and Simpson index in the gut of 3-month-old and 18-month-old Amphiprion frenatus were higher than those at other stages,showing richer species diversity.At the phylum level,the main dominant microbial groups in the intestinal tract were Proteobacteria,Firmicutes and Fusobacteria.At the genus level,the main dominant bacteria genera included Photobacterium,Pseudomonas and Achromobacter.This study can provide a theoretical support for the selection of feed for ornamental fish breeding and the development of microbial probiotic reagents.

关键词

白条双锯鱼/肠道微生物/高通量测序/观赏鱼

Key words

Amphiprion frenatus/intestinal microflora structure/high-throughput sequencing/ornamental fish

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出版年

2024

工业微生物

全国工业微生物信息中心上海市工业微生物研究所

工业微生物

影响因子：0.293

ISSN：1001-6678

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