Study on the Protective Effect of Phloretin Inclusion Complexes on ARPE-19 Cell Damage
To investigate the protective effect of Phloretin(Ph)and Phloretin/hydroxypropyl-β-cyclodextrin inclusion complex(Ph/HBC-IC)on methylglyoxal(MGO)-induced damage to human retinal pigment epithelial cells(ARPE-19),the paper prepared Ph/HBC-IC by freeze-drying method and characterized the inclusion complex using Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),X-ray diffraction(XRD)and other techniques.By using the CCK-8 method to detect cell viability,the paper screened the optimal concentration of MGO induced ARPE-19 cell damage,the optimal intervention concentrations of Ph and Ph/HBC-IC,and investigated the protective effects of Ph and Ph/HBC-IC on MGO induced ARPE-19 cell damage.The research results showed that all three characterization methods mentioned above can intuitively or indirectly prove the formation of inclusion complexes;The optimal concentration for establishing a cell damage model was 1 mmol/LMGO,and pH and pH/HBC-IC had no significant effect on cell survival within the concentration range of 0-15 mg/L.Further research has shown that after pretreatment with 15 mg/L Ph and Ph/HBC-IC,the survival rate of induced ARPE-19 cells increased by 20.4％and 28.67％,respectively,confirming that Ph and Ph/HBC-IC had a protective effect on MGO induced cell damage,and Ph/HBC-IC had a better protective effect on cells.The results provided a scientific basis for the development and applications of Ph and Ph/HBC-IC in related fields.
万方数据
维普
