Isolation and Genome Analysis of a Novel Synechococcus Cyanophage Yong-M4-211
Cyanophages can exclusively infect cyanobacteria which are regarded as an environmentally friendly biological factor with potentials to control cyanobacterial bloom.In this study,marine Synechococcus sp.7002 was used as the indicator host to isolate a novel cyanophage Yong-M4-211.It possesses a typical hexagonal head of approximately 64 nm in diameter and a flectional tail of about 112 nm in length and 7 nm in width,Siphovirus-like.The genome of Yong-M4-211 is a dsDNA genome of 53 991 bp in length with a G+C content of 67.42%.In total,93 ORFs were predicted,and no virulence factor and antibiotic resistance gene was found in the genome.Proteomic tree revealed that Yong-M4-211 presented a new family in the Caudoviricetes class.This research enlarged the database and gene library of Synechococcus cyanosiphovirus,and enriched the cognition of marine cyanophage.