Structural Characterization and Biological Properties Analysis of A Monoclonal Antibody Targeting CD47
Anti-CD47 monoclonal antibodies were expressed by transfecting Chinese hamster ovary(CHO)cell lines,and a comprehensive characterization was conducted to validate its structure and to investigate its mechanism of action.Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to confirm the molecular weight,amino acid coverage,and disulfide bond arrangement of the monoclonal antibodies,and its interaction with CD47 was studied by blocking assay.Structural confirmation showed that the measured molecular weight of the monoclonal antibodies was consistent with theoretical molecular weight,The amino acid coverage was 100％and disulfide bond pairing was consistent with the theoretical prediction.Blockade experiments demonstrated that the targeted monoclonal antibody could bind to CD47-his and block the interaction between CD47-his and signal regulatory protein alpha(SIRPα).The molecular structure of the targeted monoclonal antibodies was consistent with its theoretical design structure,which was capable of specifically binding to CD47 and blocking the CD47-SIRPα signaling axis,indicating potential anti-tumor effects.
CD47structural characterizationblocking actionultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
万方数据
维普
