Changes of cytidine triphosphate synthase expression during pulmonary inflammation in allergic asthma
Objective To investigate changes in cytidine triphosphate synthase(CTPS)in lung tissue during the sensitization and challenge phases of allergic asthma development.Methods Thirty-six BALB/c female mice were randomly divided into an ovalbumin(OVA)asthma group,an OVA sensitized group,and a healthy control group,with 12 mice in each group.The pathological changes of inflammation and mucus secretion of lung tissues were analyzed by HE and PAS staining,while the total number and classification of leukocytes in BALF were detected by a blood cell analyzer.The levels of IL-5,IL-13,and complement factor D in lung homogenates were determined by the enzyme-linked immunosorbent assay(ELISA).The CTPS mRNA and protein expression,as well as its localization in lung tissue and CD3+T cells of bronchoalveolar lavage fluid(BALF)were detected by RT-PCR,Western blot,and immunofluorescence,respectively.Results The levels of inflammation,mucus secretion,IL-5 and CFD in the lungs of mice in both the OVA sensitized and asthma groups were significantly higher than those in the healthy control group(P＜0.05),and the OVA asthma group exhibited higher levels than the OVA-sensitized group(P＜0.05).In comparison to the healthy control group,the OVA asthma group exhibited a significant increase in the number of leukocytes,neutrophils,lymphocytes,monocytes,and eosinophils in BALF,as well as an elevated expression of IL-13 in lung homogenates(P＜0.05).Additionally,the expression of CTPS protein was significantly increased in the lung tissues,airway epithelial cells,cells in the inflammatory areas of the lungs,and CD3+T cells in the BALF(P＜0.05),in which some of the cells displayed distinctive"cellular snake"structures,including rods,bars,"C"-shaped or crescent-shaped CTPS proteins.Conclusion The CTPS protein was significantly elevated only in the OVA-induced asthma phase,and its augmented expression and the formation of"cellular snake"structures may be closely related to the rise of inflammatory cells,particularly CD3+T cells,within the pulmonary region.
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