Study on the mechanism of apoptosis of human bronchial epithelial cells induced by nickel sulfate
Objective To study the toxic effect and injury mechanism on human bronchial epithelial cells(Beas-2B)by NiSO4,and to explore the role of the Wnt/β-catenin pathway in it.Methods Different concentrations of NiSO4(0,50,100,200 μmol/L)were selected to treat Beas-2B cells for 24 hours,the cytotoxicity of NiSO4 was detected by CCK-8 assay,and the contents of malondialdehyde and total iron ions in cells were detected by lipid peroxidation mda assay kit and total iron content colorimetric assay kit.Western blot was used to detect the expression of Wnt3a,β-catenin,Ferroptosis related proteins GPX4,xCT,Apoptosis related proteins Bcl-2,Bax.Beas-2B cells were treated with XAV-939,a pathway inhibitor,and then treated with 100 μmol/L NiSO4 for 24 h.The expression levels of GPX4 and xCT were detected.Results With the increase of NiSO4 concentration,Beas-2B cell survival rate significantly reduced(F=12.530,P＜0.05),the contents of malondialdehyde and total iron ion decreased,the expression of Wnt3a,β-catenin gradually increased(F=9.209,3.936,7.639,23.350,P＜0.05),the expression of GPX4,xCT increased,the expression of Bax gradually increased and the expression of Bcl-2 decreased gradually(F=6.903,17.760,52.930,14.460,P＜0.05).Compared with 100 μmol/L group,the expression of Wnt3a,β-catenin,GPX4 and xCT in Beas-2B cells treated with XAV-939 decreased(t=8.055,8.049,9.659,11.70,P＜0.05).Conclusion In vitro experiments showed that NiSO4 can induce apoptosis in Beas-2B cells while possibly activating Wnt/β-catenin pathway inhibits cell ferroptosis.
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