Effects of pre-induction of HSP90α on the heat stress in HepG2 cells
Objective To investigate the responses to heat stress in cells with pre-induction of HSP90α,and explore the strategy to induce thermal acclimation of cells.Methods Human hepatocellular carcinoma cell line HepG2 was cultured at 42 ℃ to create a heat stress model.Expression of HSP90α was pre-induced by 17-DMAG,an HSP90 inhibitor,at a concentration that does not damage cell viability.Reactive oxygen species(ROS)and apoptosis were detected by flow cytometry.Cell viability was detected by CCK-8,and protein changes were detected by Western blot.Results After heat shock for 0.5-4 h,the content of intracellular ROS gradually increased(F=332.22,P＜0.01).After recovery to 24 h at 37 ℃,more γH2AX was observed in the 1-4 h heat shock group.Meanwhile,cell viabilities were attenuated as heat shock prolonged(F=31.94,P＜0.01),and 4 h heat shock decreased cell viability to(71.39±2.55)％of control group.After the expression of HSP90α was pre-induced by 5 nmol/L 17-DMAG for 24 h,heat shock for 4 h at 42 ℃ followed by a recovery at 37 ℃ to 24 h,yH2AX was decreased compared with cells without HSP90α pre-induction;and the apoptosis rate of cells were also decreased,from(26.06±2.48)％to(15.93±1.67)％(F=62.79,P＜0.01).Conclusion Pre-induction of HSP90a can reduce cells'DNA damage and apoptosis after heat stress,help-ing cells obtain rapid heat acclimation.
Heat stressOxidative stressPre-induction of HSP90αHeat acclimation
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