Correlation between long working hours and expression of DNA adducts εdA and εdC levels
Objective To study the effect of working hours on Etheno-DNA adduct(edA,edC)levels in nurses'peripheral blood.Methods In this study,a cross-sectional survey was used to recruit female nurses as the study subjects from a total of 12 hospitals in four regions,including Qingyuan City in Guangdong Province,Changsha City in Hunan Province,Urumqi City in Xinjiang Uygur Autonomous Region,and Chongqing Municipality,from 2022 to 2024.Inclusion criteria were:(1)healthy,no history of underlying diseases;(2)on-the-job clinical registered nurses who have been engaged in nursing for more than one year;(3)not older than 55 years;(4)no history of long-term smoking and alcohol consumption;(5)normal menstrual cycle;(6)consent to participate in the study and sign the informed consent voluntarily.Exclusion criteria were:(1)maternal;(2)history of breast cancer,or family history of breast cancer in the immediate family(parents,children,brothers and sisters);(3)clinically diagnosed diabetes mellitus,hypertension,hyperlipidemia,cardiovascular disease,other types of tumors,renal disease,chronic pain;(4)taking melatonin-containing supplements,immunosuppressants,hormonal drugs,hypogly-cemic drugs,lipid-lowering drugs,or steroids in the past three months,lipid-lowering or steroid medications,and antioxi-dant supplements;(5)ICU,radiology,or emergency department;and(6)long-term sick leave.An on-site question-naire was used to collect the basic information of the study participants,such as working hours,age,length of service,and health characteristics.Grouped according to working hours,including 25 people in the standard working hours group(35-40 h/week),36 people in the 41-48 h/week group,and 30 people in the ≥49 h/week group in four groups,a total of 91 study subjects were included.Five ml of pre-shift fasting venous blood was collected from each study subject,and the levels of Etheno-DNA adducts(εdA,εdC)in the peripheral blood of the study subjects were detected by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS-MS).The effects of age,length of service,and health characteristics on the levels of Etheno-DNA adducts(εdA,εdC)were explored by univariate analysis of vari-ance(ANOVA).Multiple linear regression modeling was used to test the associations between nurses'working hours and the levels of Etheno-DNA adducts(εdA,εdC)after balancing out the confounding factors that might affect the levels of adducts.Results Blood levels of εdA and εdC adducts were significantly higher(ZεdC=2.424,ZεdA=1.776;P＜0.05)in study subjects in the ≥49 h/week group compared with the standard working-hours group(35-40 h/week);Multiple linear regression showed that after balancing for possible confounders(e.g.,age,length of service,health characteristics),whole blood levels of Etheno-DNA adducts(εdA and εdC)were significantly higher(P＜0.05)in the ≥49 h/week group compared with the standard working-hours group(Etheno-DNA adduct(εdA,εdC)levels were significantly higher in whole blood in the working hours ≥49 h/week group compared to the standard working-hours group(35-40 h/week)(β=0.70,0.41,P＜0.05).Conclusion Long working hours are one of the risk factors for elevated levels of Etheno-DNA adducts(εdA,εdC)in peripheral blood.Nurses'peripheral blood levels of Etheno-DNA adducts(εdA,ε dC)were significantly increased when working more than the standard number of hours(35-40 h/week),especially when working ≥49 h/week.
Long working hoursOxidative stressLipid peroxidationEtheno-DNA adducts
NETL
NSTL
万方数据
