Effects of lead poisoning on intestinal microbe and barrier function in rats
Objective To investigate the effects of lead poisoning on intestinal flora and intestinal barrier function in rats,and to provide new methods for preventing lead poisoning.Methods Three-week-old healthy Wistar male rats were randomly divided into the control and model groups(6 rats for each group).Lead acetate solution(30 mg/kg body weight)was injected intraperitoneally once every other day for 14 days.After that,the serum levels of D-lactate and diamine oxidase(DAO)were detected.The morphology of ileum was observed by HE staining.The content of zonula occludens-1(ZO-1)was detected by immunohistochemical staining.The 16S rRNA sequencing was used to detect the relative abundance of microbes in the ileum.Results The results showed that lead poisoning changed the morphology of intestinal epithelial cells,decreased the level of ZO-1,and destroyed the mucosal barrier.Serum D-lactic acid(396.0±9.9 ng/mL)and DAO(5.4±0.1 mg/mL)levels in lead poisoning group were significantly higher than those in control group(D-lactic acid:382.0±7.8 ng/mL,DAO:5.0±0.2 mg/mL,P＜0.05).Lead poisoning reduced the diversity of microbe in rats,and the relative abundance of Romboutsia,Clostridium,Turicibacter,Corynebacterium and Oligella deereased,while Escherichia-Shigella and Bacteroides became the primary characteristic microbe.Conclusion Lead poisoning could damage the intestinal barrier function and alter the intestinal flora.
万方数据
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