The Effect of Different Pressure Stimulation in Vitro on the Expression of Integrin β1 Subunit and Microfilaments in Fascia Fibroblasts Related to Meridians
Objective: To provide experimental evidence of cell biology principle in basic needle push mechanics through in itvro study of the influence of stress stimulation to the transduction pathway of integrin β1 subunit and cytoskeleton microfilaments mechanical signal in fibroblasts of meridian fascia connective tissue.Methods: The fibroblasts cells of meridian fascia connective tissue were cultured in vitro and stimulated by single or multiple stimulation of different intensity of pressure.Then the expression of integrin β1 subunit in each group were detected and the morphological changes in cytoskeleton microfilaments of fibroblasts were observed.Results: (1) Meridians fascia connective tissue fibroblasts could feel the stimulation through integrin β1.When stimulated by single moderate and severe intensity, the integrin β1 in fibroblasts presented the highest expression level.However, when stimulated by repeated light intensity, the expression of integrin β1 reached the highest level.Analysis of variance showed that the number and intensity of stimulation were both factors that causing β1 integrin change (P ＜ 0.01).And there was an interaction of two factors (P ＜ 0.01).(2) The cytoskeleton microfilaments of fibroblasts would change after meridians related fascia connective tissue fibroblasts were stimulated by different stress.Whether single or multiple stimulation were applied, the morphological of microfilaments both showed a gradient change, and the gradient feature in the multiple stimulation group were much more obvious than in the single stimulation group.Conclusion: The phenomenon of integrin β1 expression and morphological change of microfilanents in fascia fibroblasts related to the meridians during in vitro stimulation may reveal, from the point of cell mechanics, the mechanisms of biophysical signal feeling and transduction in acupuncture push stress stimulation to meridians related tissue cells.
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