目的:建立高效液相色谱法测定复方足癣乳膏中甲硝唑的含量.方法:色谱柱:DIKMA Diamonsil C 18柱(250×4.6mm,5 μm),流动相:甲醇-水(20∶80);流速:1.0mL·min-1,柱温:30℃,检测波长:320nm.结果:甲硝唑在3.148μg.mL-1～37.78μg.mL-1范围内线性关系良好(r=0.9995),平均回收率100.80％,RSD =2.5％.结论:本方法结果准确,专属性强,重复性好,可用于复方足癣乳膏的含量测定方法.
Determination of the Metronidazole in Tinea pedis Cream by HPLC
Objective:To establish an HPLC method for the determination of metronidazole in Tinea pedis cream.Method:The column was D1KMA DiamonsilC18 (250mm × 4.6mm,5μm).The mobile phase was methanol-water(20∶80).The flow rate was 1.0 mL · min-1 The detection wavelength was 320nm and the temperature of column was 30℃.Result:The metronidazole showed a good linearity in the range of 3.148-37.78μg.mL-1,r =0.9995.The average recovery was 100.80％,and RSD was 2.5％.Conclusion:This method is simple,accurate and reliable,and can be used for the quality of Tinea pedis cream.
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